Single colonies of some fungal species can colonize massive areas as large as five soccer stadia. The ubiquitous fungus Aspergillus niger grows radially, forming long hyphae penetrating the substrate. It is an industrial fungus producing a various range of food additives but it is also responsible for food spoilage by causing the so-called black mold disease. Grown on sugar beet pulp, the by-product of sugar industry, we showed how different the response of different parts of one colony of this fungus are, depending on the availability of nutrients. A. niger is able to turn on the production of different enzyme sets in different parts of its colony. Like a Swiss army knife, the fungus uses its enzymes to cut the polymeric structure of the sugar beet pulp and release simple sugars. In older parts of the colony, some components of sugar beet pulp have already been used and therefore different enzymes are needed for the substrate that has remained. In biotechnology these enzymes can be used to release sugars from plant biomass that can be used for bioethanol production. The flexibility of A. niger to produce different enzyme sets makes it highly useful in producing enzymes for a large range of plant biomass substrates, allowing also flexibility in the biotechnological process of biofuel production.