Popular plasmids
Some of our most
popular plasmids:
pGEX1, 2T and 3X improved versions: pRP* plasmids
- Expression of DNA sequences fused to the 3' end of the Sj26 (glutathione-S-transferase)
gene: fusion proteins
are soluble in aqeous solutions, isolation procedure of fusion
protein is simple and rapid, high yields can be achieved, site-specific proteolysis
of fusion proteins possible by thrombin or factor Xa.
pMPM plasmids:
- Vectors with combinations of different drug selection markers, plasmid copy numbers
(low, medium or high), compatible with each other and some having an expression
cassette which allows high level, inexpensive, extremely tight regulation of expression
(based on the promoter and AraC repressor/activator of the ara operon).
Sequences available.
pBBR1MCS derivatives:
- Improved and useful broad host range cloning vectors: small, mobilizable vectors, compatible with numerous plasmid replicons (IncP, Q and W, ColE1 and p15A), possessing
an extended mcs, direct selection of recombinant plasmid possible in E. coli.
Sequences available.
pT7* and pEB*, some pET*, pLysE and pLysS plasmids
- Expression from a T7 promoter (as well E. coli as broad host range plasmids).
pKNG101:
- For introducing a single copy of a cloned gene stably into the chromosome of a
wide range of bacterial species (Gram-negative), positive selection of double recombinant
events possible.
pBSL plasmids:
- pBSL14, 15 and 86
Kanamycin resistance gene cassettes with flanking polylinkers (in either direct
or inverted orientation): can be used for vector construction (a convenient source
of the Km resistance determinant) and in vitro insertion/deletion mutagenesis
of cloned DNA fragments. Can also be used as a source of restriction sites and for
restriction site mobilization (positive selection possible, introduction of polylinker
and selectable marker simultaneously, simple determination of orientation).
Sequences available.
- pBSL118, 202, 203, 204, 299
Mini-Tn5 derivatives encoding resistance to Km, Cm, Gm, Tc and Sm coupled
with the polylinker of pBluescriptII: for insertion mutagenesis and genetic engineering
in Gram-negative bacteria.
- pBSL 97, 99, 119, 121, 128, 130, 141, 168, 175, 190, 193, 142 and 159
Plasmids carrying improved antibiotic resistance gene cassettes and omega elements
for vector construction and in vitro deletion/insertion mutagenesis.
Sequences available.
- pBSL180, 181, 182 and 199
Mini-Tn10 transposon derivatives for insertion mutagenesis and gene delivery into
the chromosome of Gram-negative bacteria.
- pBSL63, 237 and 238
RP4 oriT and RP4 oriT-R6K oriV DNA cassettes for construction
of specialized vectors.
pKNOCK plasmids:
- Broad host range, mobilizable suicide vectors for gene knockout and targeted DNA
insertion into the chromosome of Gram-negative bacteria.